In the present study, two hyper hydrolytic enzyme producer mutant strains i.e. B.stratosphericus N12 (M) and B. altitudinis Kd1 (M)have been used for enzyme production which exhibited an appreciable increase i.e. 160.49% in cellulase and 105.80% in xylanase activities over their respective wild strains. The already optimized conditions of OFAT were opted further to switch over to more efficient statistical tool i.e. Response surface Methodology (RSM). Significant increase in reducing sugars i.e. 33.21 mg/g with overall 453.50 % through RSM was achieved. Quantitative analysis of sugars by using High Performance Liquid Chromatography (HPLC) technique has also been done.